NanoBio:Protocols
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==Sub-cloning using Biobricks or Biofusion Vectors== | ==Sub-cloning using Biobricks or Biofusion Vectors== | ||
*Note: Many of these are protocols that I used and/or developed as a postdoctoral fellow in Pam Silver's lab. | *Note: Many of these are protocols that I used and/or developed as a postdoctoral fellow in Pam Silver's lab. | ||
| - | *[[NanoBio: | + | *[[NanoBio: Biobrick/Biofusion Strategy | Strategy for Making Parts]] |
*[[NanoBio: Designing Parts or Genes| Designing Parts or Genes ]] | *[[NanoBio: Designing Parts or Genes| Designing Parts or Genes ]] | ||
*[[NanoBio: Commonly Used Plasmids | Commonly Used Plasmids]] | *[[NanoBio: Commonly Used Plasmids | Commonly Used Plasmids]] | ||
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*[[NanoBio: Site-Directed Mutagenesis | Site-Directed Mutagenesis]] | *[[NanoBio: Site-Directed Mutagenesis | Site-Directed Mutagenesis]] | ||
*[[NanoBio: Restriction Digest | Restriction Digest]] | *[[NanoBio: Restriction Digest | Restriction Digest]] | ||
| - | |||
*[[Silver: Plasmid Verification | Plasmid Verification]] | *[[Silver: Plasmid Verification | Plasmid Verification]] | ||
*[[NanoBio: Ligation | Ligation]] | *[[NanoBio: Ligation | Ligation]] | ||
Revision as of 22:20, 29 January 2008
Contents |
Nano Synth Bio
General Molecular Biology
Current Protocols in Molecular Biology
Sub-cloning using Biobricks or Biofusion Vectors
- Note: Many of these are protocols that I used and/or developed as a postdoctoral fellow in Pam Silver's lab.
- Strategy for Making Parts
- Designing Parts or Genes
- Commonly Used Plasmids
- Commonly Used Primers
- PCR
- Oligonucleotide Inserts
- Overlapping Oligonucleotide Inserts
- Site-Directed Mutagenesis
- Restriction Digest
- Plasmid Verification
- Ligation
- 3 Antibiotic Assembly
- Bacterial Transformation
- Plasmid Verification
Genomic Integration into S. cerevisiae
Lipid Bilayers
Care of Synechocystis sp 6803
Primers
- Biobricks & Biofusion Sub-cloning
- Synechocystis sp. 6803
- Shewanella oneidensis MR-1
- Guidelines on Talking or Writing about your project
- Microbial Respiration
