NanoBio:Protocols: Difference between revisions
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[http://www.mrw.interscience.wiley.com/cp/cpmb/cpmb_contents_fs.html Current Protocols in Molecular Biology] | [http://www.mrw.interscience.wiley.com/cp/cpmb/cpmb_contents_fs.html Current Protocols in Molecular Biology] | ||
==Sub-cloning | ==Sub-cloning == | ||
*[[NanoBio: Biobrick/fusion Strategy | | *[[NanoBio: Biobrick/fusion Strategy | BioBrick & Biofusion Strategies for Making Parts]] | ||
*[[NanoBio: Designing Parts or Genes| Designing Parts or Genes ]] | *[[NanoBio: Designing Parts or Genes| Designing Parts or Genes ]] | ||
*[[NanoBio: Commonly Used Plasmids | Commonly Used Plasmids]] | *[[NanoBio: Commonly Used Plasmids | Commonly Used Plasmids]] | ||
Revision as of 12:13, 27 March 2008
Nano Synth Bio
General Molecular Biology
Current Protocols in Molecular Biology
Sub-cloning
- BioBrick & Biofusion Strategies for Making Parts
- Designing Parts or Genes
- Commonly Used Plasmids
- Commonly Used Primers
- PCR
- Oligonucleotide Inserts
- Overlapping Oligonucleotide Inserts
- Site-Directed Mutagenesis
- Restriction Digest
- Ligation
- 3 Antibiotic Assembly
- Bacterial Transformation
- Plasmid Verification
- Note: Many of these are protocols that I used and/or developed as a postdoctoral fellow in Pam Silver's lab.
Genomic Integration into S. cerevisiae
Lipid Bilayers
Care of Synechocystis sp 6803
Primers
- Biobricks & Biofusion Sub-cloning
- Synechocystis sp. 6803
- Shewanella oneidensis MR-1
- Guidelines on Talking or Writing about your project
- Microbial Respiration
