NanoBio:Protocols
From OpenWetWare
(Difference between revisions)
(→Sub-cloning) |
|||
| Line 20: | Line 20: | ||
*[[NanoBio: Bacterial Transformation | Bacterial Transformation]] | *[[NanoBio: Bacterial Transformation | Bacterial Transformation]] | ||
*[[NanoBio: Plasmid Verification | Plasmid Verification]] | *[[NanoBio: Plasmid Verification | Plasmid Verification]] | ||
| - | * | + | |
| + | ==Protein Expression & Purification == | ||
| + | *[[NanoBio: Bacterial Hosts for Overexpression|Bacterial Hosts for Overexpression]] | ||
| + | *[[NanoBio: Test Induction & Purification|Test Induction & Purification]] | ||
| + | *[[NanoBio: Protein Purification|Protein Purification]] | ||
==Genomic Integration into S. cerevisiae== | ==Genomic Integration into S. cerevisiae== | ||
Revision as of 13:04, 13 June 2008
Contents |
Nano Synth Bio
General Molecular Biology
Current Protocols in Molecular Biology
Sub-cloning
- Introduction to Cloning A nice introduction to cloning, written by Chris Anderson
- Introduction to BioBricks A nice introduction to BioBrick cloning, written by Chris Anderson
- Installing Vector NTI How to install Vector NTI, a free sequence editor
- BioBrick & Biofusion Strategies for Making Parts
- Designing Parts or Genes
- Commonly Used Plasmids
- Commonly Used Primers
- PCR
- Oligonucleotide Inserts
- Overlapping Oligonucleotide Inserts
- Site-Directed Mutagenesis
- Restriction Digest
- Ligation
- 3 Antibiotic Assembly
- Bacterial Transformation
- Plasmid Verification
Protein Expression & Purification
Genomic Integration into S. cerevisiae
Lipid Bilayers
Care of Synechocystis sp 6803
Primers
- Biobricks & Biofusion Sub-cloning
- Synechocystis sp. 6803
- Shewanella oneidensis MR-1
- Guidelines on Talking or Writing about your project
- Microbial Respiration
