NanoBio:Protocols
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=Nano Synth Bio= | =Nano Synth Bio= | ||
==General Molecular Biology== | ==General Molecular Biology== | ||
| - | [http://www.mrw.interscience.wiley.com/cp/cpmb/ | + | [http://www.mrw.interscience.wiley.com/emrw/9780471142720/cp/cpmb/toc Current Protocols in Molecular Biology] |
| - | ==Sub-cloning | + | ==Lab Practices== |
| - | *[[NanoBio: Biobrick/fusion Strategy | | + | *[[NanoBio: Notebook| Keeping a Laboratory Notebook]] |
| - | *[[ | + | |
| + | ==Sub-cloning == | ||
| + | *[[Arking:JCATutorialIntro7| Introduction to Cloning]] A nice introduction to cloning, written by Chris Anderson | ||
| + | *[[Arking:JCATutorialIntro1| Introduction to BioBricks]] A nice introduction to BioBrick cloning, written by Chris Anderson | ||
| + | *[[NanoBio: Installing Vector NTI| Installing Vector NTI]] How to install Vector NTI, a free sequence editor | ||
| + | *[[NanoBio: Biobrick/fusion Strategy | BioBrick & Biofusion Strategies for Making Parts]] An overview of how to make & combine parts. | ||
| + | *[[Synthetic_Biology:BioBricks/3A_assembly| 3 Antibiotic Assembly]] | ||
*[[NanoBio: Commonly Used Plasmids | Commonly Used Plasmids]] | *[[NanoBio: Commonly Used Plasmids | Commonly Used Plasmids]] | ||
*[[NanoBio: Commonly Used Primers | Commonly Used Primers]] | *[[NanoBio: Commonly Used Primers | Commonly Used Primers]] | ||
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*[[NanoBio: Site-Directed Mutagenesis | Site-Directed Mutagenesis]] | *[[NanoBio: Site-Directed Mutagenesis | Site-Directed Mutagenesis]] | ||
*[[NanoBio: Restriction Digest | Restriction Digest]] | *[[NanoBio: Restriction Digest | Restriction Digest]] | ||
| - | |||
*[[NanoBio: Ligation | Ligation]] | *[[NanoBio: Ligation | Ligation]] | ||
| - | *[[NanoBio: | + | *[[NanoBio: Bacterial Transformation | Bacterial Transformation of Chemically Competent Cells]] |
| - | *[[NanoBio: Bacterial Transformation | Bacterial Transformation]] | + | *[[NanoBio: Prep for Electrocompetent cells | Prep for Electrocompetent cells]] |
| + | *[[NanoBio: Bacterial Transformation using Electroporation | Bacterial Transformation using Electroporation]] | ||
*[[NanoBio: Plasmid Verification | Plasmid Verification]] | *[[NanoBio: Plasmid Verification | Plasmid Verification]] | ||
| - | * | + | *[[NanoBio: AgaroseGels | Agarose Gels]] |
| + | |||
| + | ==Protein Expression & Purification == | ||
| + | *[[NanoBio: Bacterial Hosts for Overexpression|Bacterial Hosts for Overexpression]] | ||
| + | *[[NanoBio: Test Induction & Purification|Test Induction & Purification]] | ||
| + | *[[NanoBio: Protein Purification|Protein Purification]] | ||
| + | *[[NanoBio: Isolation of Periplasmic Fraction|Isolation of Periplasmic Fraction]] | ||
| + | *[[NanoBio: Protein Gels|Protein Gels]] | ||
| + | *[[NanoBio: Cell Surface Binding Assay|Cell Surface Binding Assay]] | ||
| + | |||
| + | ==Gene deletion with λ-red == | ||
| + | *[[NanoBio: Overview and Materials for λ-red|Overview and Materials for λ-red]] | ||
| + | *[[NanoBio: Primer Design|Primer Design]] | ||
| + | *[[NanoBio: Protocol for gene knockout|Protcol for gene knockout]] | ||
| + | *[[NanoBio: Knockout/in|Knockout/in]] | ||
==Genomic Integration into S. cerevisiae== | ==Genomic Integration into S. cerevisiae== | ||
| - | *[[ | + | *[[NanoBio: Integration into yeast | Integration into Yeast]] |
| - | *[[ | + | *[[NanoBio: Verifying Genomic Integration | Verifying Genomic Integration]] |
==Lipid Bilayers== | ==Lipid Bilayers== | ||
*[[NanoBio: Making Unilamellar Vesicles| Making Unilamellar Vesicles]] | *[[NanoBio: Making Unilamellar Vesicles| Making Unilamellar Vesicles]] | ||
*[[NanoBio: Preparation of Supported Bilayer| Preparation of Supported Bilayers]] | *[[NanoBio: Preparation of Supported Bilayer| Preparation of Supported Bilayers]] | ||
| + | *[[NanoBio: Cleaning SiO2 surfaces| Cleaning SiO2 surfaces]] | ||
| + | |||
==Care of Synechocystis sp 6803== | ==Care of Synechocystis sp 6803== | ||
*[[NanoBio: Liquid & Solid Growth Media| Liquid & Solid Growth Media]] | *[[NanoBio: Liquid & Solid Growth Media| Liquid & Solid Growth Media]] | ||
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* [[NanoBio: BG-11 media | BG-11 media]] | * [[NanoBio: BG-11 media | BG-11 media]] | ||
* [[NanoBio: BG-11 plates| BG-11 plates]] | * [[NanoBio: BG-11 plates| BG-11 plates]] | ||
| + | * [[NanoBio: Supplemented M9 Media| Supplemented M9 Media]] | ||
==Safety & Waste Disposal== | ==Safety & Waste Disposal== | ||
*[[NanoBio: Safety & Waste Disposal | Safety & Waste Disposal]] | *[[NanoBio: Safety & Waste Disposal | Safety & Waste Disposal]] | ||
Revision as of 17:36, 11 June 2010
Contents |
Nano Synth Bio
General Molecular Biology
Current Protocols in Molecular Biology
Lab Practices
Sub-cloning
- Introduction to Cloning A nice introduction to cloning, written by Chris Anderson
- Introduction to BioBricks A nice introduction to BioBrick cloning, written by Chris Anderson
- Installing Vector NTI How to install Vector NTI, a free sequence editor
- BioBrick & Biofusion Strategies for Making Parts An overview of how to make & combine parts.
- 3 Antibiotic Assembly
- Commonly Used Plasmids
- Commonly Used Primers
- PCR
- Oligonucleotide Inserts
- Overlapping Oligonucleotide Inserts
- Site-Directed Mutagenesis
- Restriction Digest
- Ligation
- Bacterial Transformation of Chemically Competent Cells
- Prep for Electrocompetent cells
- Bacterial Transformation using Electroporation
- Plasmid Verification
- Agarose Gels
Protein Expression & Purification
- Bacterial Hosts for Overexpression
- Test Induction & Purification
- Protein Purification
- Isolation of Periplasmic Fraction
- Protein Gels
- Cell Surface Binding Assay
Gene deletion with λ-red
Genomic Integration into S. cerevisiae
Lipid Bilayers
Care of Synechocystis sp 6803
Primers
- Biobricks & Biofusion Sub-cloning
- Synechocystis sp. 6803
- Shewanella oneidensis MR-1
- Guidelines on Talking or Writing about your project
- Microbial Respiration
