NanoBio:Protocols
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==General Molecular Biology== | ==General Molecular Biology== | ||
[http://www.mrw.interscience.wiley.com/emrw/9780471142720/cp/cpmb/toc Current Protocols in Molecular Biology] | [http://www.mrw.interscience.wiley.com/emrw/9780471142720/cp/cpmb/toc Current Protocols in Molecular Biology] | ||
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| + | ==Lab Practices== | ||
| + | *[[NanoBio: Notebook| Keeping a Laboratory Notebook]] | ||
==Sub-cloning == | ==Sub-cloning == | ||
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*[[NanoBio: Ligation | Ligation]] | *[[NanoBio: Ligation | Ligation]] | ||
*[[NanoBio: Bacterial Transformation | Bacterial Transformation of Chemically Competent Cells]] | *[[NanoBio: Bacterial Transformation | Bacterial Transformation of Chemically Competent Cells]] | ||
| + | *[[NanoBio: Prep for Electrocompetent cells | Prep for Electrocompetent cells]] | ||
*[[NanoBio: Bacterial Transformation using Electroporation | Bacterial Transformation using Electroporation]] | *[[NanoBio: Bacterial Transformation using Electroporation | Bacterial Transformation using Electroporation]] | ||
*[[NanoBio: Plasmid Verification | Plasmid Verification]] | *[[NanoBio: Plasmid Verification | Plasmid Verification]] | ||
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*[[NanoBio: Test Induction & Purification|Test Induction & Purification]] | *[[NanoBio: Test Induction & Purification|Test Induction & Purification]] | ||
*[[NanoBio: Protein Purification|Protein Purification]] | *[[NanoBio: Protein Purification|Protein Purification]] | ||
| + | *[[NanoBio: Isolation of Periplasmic Fraction|Isolation of Periplasmic Fraction]] | ||
*[[NanoBio: Protein Gels|Protein Gels]] | *[[NanoBio: Protein Gels|Protein Gels]] | ||
*[[NanoBio: Cell Surface Binding Assay|Cell Surface Binding Assay]] | *[[NanoBio: Cell Surface Binding Assay|Cell Surface Binding Assay]] | ||
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==Gene deletion with λ-red == | ==Gene deletion with λ-red == | ||
*[[NanoBio: Overview and Materials for λ-red|Overview and Materials for λ-red]] | *[[NanoBio: Overview and Materials for λ-red|Overview and Materials for λ-red]] | ||
| + | *[[NanoBio: Primer Design|Primer Design]] | ||
*[[NanoBio: Protocol for gene knockout|Protcol for gene knockout]] | *[[NanoBio: Protocol for gene knockout|Protcol for gene knockout]] | ||
| + | *[[NanoBio: Knockout/in|Knockout/in]] | ||
==Genomic Integration into S. cerevisiae== | ==Genomic Integration into S. cerevisiae== | ||
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*[[NanoBio: Making Unilamellar Vesicles| Making Unilamellar Vesicles]] | *[[NanoBio: Making Unilamellar Vesicles| Making Unilamellar Vesicles]] | ||
*[[NanoBio: Preparation of Supported Bilayer| Preparation of Supported Bilayers]] | *[[NanoBio: Preparation of Supported Bilayer| Preparation of Supported Bilayers]] | ||
| + | *[[NanoBio: Cleaning SiO2 surfaces| Cleaning SiO2 surfaces]] | ||
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==Care of Synechocystis sp 6803== | ==Care of Synechocystis sp 6803== | ||
*[[NanoBio: Liquid & Solid Growth Media| Liquid & Solid Growth Media]] | *[[NanoBio: Liquid & Solid Growth Media| Liquid & Solid Growth Media]] | ||
Revision as of 17:36, 11 June 2010
Contents |
Nano Synth Bio
General Molecular Biology
Current Protocols in Molecular Biology
Lab Practices
Sub-cloning
- Introduction to Cloning A nice introduction to cloning, written by Chris Anderson
- Introduction to BioBricks A nice introduction to BioBrick cloning, written by Chris Anderson
- Installing Vector NTI How to install Vector NTI, a free sequence editor
- BioBrick & Biofusion Strategies for Making Parts An overview of how to make & combine parts.
- 3 Antibiotic Assembly
- Commonly Used Plasmids
- Commonly Used Primers
- PCR
- Oligonucleotide Inserts
- Overlapping Oligonucleotide Inserts
- Site-Directed Mutagenesis
- Restriction Digest
- Ligation
- Bacterial Transformation of Chemically Competent Cells
- Prep for Electrocompetent cells
- Bacterial Transformation using Electroporation
- Plasmid Verification
- Agarose Gels
Protein Expression & Purification
- Bacterial Hosts for Overexpression
- Test Induction & Purification
- Protein Purification
- Isolation of Periplasmic Fraction
- Protein Gels
- Cell Surface Binding Assay
Gene deletion with λ-red
Genomic Integration into S. cerevisiae
Lipid Bilayers
Care of Synechocystis sp 6803
Primers
- Biobricks & Biofusion Sub-cloning
- Synechocystis sp. 6803
- Shewanella oneidensis MR-1
- Guidelines on Talking or Writing about your project
- Microbial Respiration
