NanoBio:Protocols

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Protein Expression & Purification)
(Sub-cloning)
(11 intermediate revisions not shown.)
Line 2: Line 2:
==General Molecular Biology==
==General Molecular Biology==
[http://www.mrw.interscience.wiley.com/emrw/9780471142720/cp/cpmb/toc Current Protocols in Molecular Biology]
[http://www.mrw.interscience.wiley.com/emrw/9780471142720/cp/cpmb/toc Current Protocols in Molecular Biology]
 +
 +
==Lab Practices==
 +
*[[NanoBio: Notebook| Keeping a Laboratory Notebook]]
==Sub-cloning ==
==Sub-cloning ==
Line 17: Line 20:
*[[NanoBio: Restriction Digest | Restriction Digest]]
*[[NanoBio: Restriction Digest | Restriction Digest]]
*[[NanoBio: Ligation | Ligation]]
*[[NanoBio: Ligation | Ligation]]
-
*[[NanoBio: Bacterial Transformation | Bacterial Transformation]]
+
*[[NanoBio: Bacterial Transformation | Bacterial Transformation of Chemically Competent Cells]]
 +
*[[NanoBio: Prep for Electrocompetent cells | Prep for Electrocompetent cells]]
 +
*[[NanoBio: Bacterial Transformation using Electroporation | Bacterial Transformation using Electroporation]]
*[[NanoBio: Plasmid Verification | Plasmid Verification]]
*[[NanoBio: Plasmid Verification | Plasmid Verification]]
 +
*[[NanoBio: Archiving Your Plasmid in the Strain Collection | Archiving Your Plasmid in the Strain Collection]]
*[[NanoBio: AgaroseGels | Agarose Gels]]
*[[NanoBio: AgaroseGels | Agarose Gels]]
Line 25: Line 31:
*[[NanoBio: Test Induction & Purification|Test Induction & Purification]]
*[[NanoBio: Test Induction & Purification|Test Induction & Purification]]
*[[NanoBio: Protein Purification|Protein Purification]]
*[[NanoBio: Protein Purification|Protein Purification]]
 +
*[[NanoBio: Isolation of Periplasmic Fraction|Isolation of Periplasmic Fraction]]
*[[NanoBio: Protein Gels|Protein Gels]]
*[[NanoBio: Protein Gels|Protein Gels]]
 +
*[[NanoBio: Cell Surface Binding Assay|Cell Surface Binding Assay]]
 +
 +
==Gene deletion with λ-red ==
 +
*[[NanoBio: Overview and Materials for λ-red|Overview and Materials for λ-red]]
 +
*[[NanoBio: Primer Design|Primer Design]]
 +
*[[NanoBio: Protocol for gene knockout|Protcol for gene knockout]]
 +
*[[NanoBio: Knockout/in|Knockout/in]]
==Genomic Integration into S. cerevisiae==
==Genomic Integration into S. cerevisiae==
Line 34: Line 48:
*[[NanoBio: Making Unilamellar Vesicles| Making Unilamellar Vesicles]]
*[[NanoBio: Making Unilamellar Vesicles| Making Unilamellar Vesicles]]
*[[NanoBio: Preparation of Supported Bilayer| Preparation of Supported Bilayers]]
*[[NanoBio: Preparation of Supported Bilayer| Preparation of Supported Bilayers]]
 +
*[[NanoBio: Cleaning SiO2 surfaces| Cleaning SiO2 surfaces]]
 +
==Care of Synechocystis sp 6803==
==Care of Synechocystis sp 6803==
*[[NanoBio: Liquid & Solid Growth Media| Liquid & Solid Growth Media]]
*[[NanoBio: Liquid & Solid Growth Media| Liquid & Solid Growth Media]]
Line 51: Line 67:
* [[NanoBio: BG-11 media | BG-11 media]]
* [[NanoBio: BG-11 media | BG-11 media]]
* [[NanoBio: BG-11 plates| BG-11 plates]]
* [[NanoBio: BG-11 plates| BG-11 plates]]
 +
* [[NanoBio: Supplemented M9 Media| Supplemented M9 Media]]
==Safety & Waste Disposal==
==Safety & Waste Disposal==
*[[NanoBio: Safety & Waste Disposal | Safety & Waste Disposal]]
*[[NanoBio: Safety & Waste Disposal | Safety & Waste Disposal]]

Revision as of 15:53, 21 March 2013

Contents

Nano Synth Bio

General Molecular Biology

Current Protocols in Molecular Biology

Lab Practices

Sub-cloning

Protein Expression & Purification

Gene deletion with λ-red

Genomic Integration into S. cerevisiae

Lipid Bilayers

Care of Synechocystis sp 6803

Primers

Recipes

Safety & Waste Disposal

Personal tools