NanoBio:Protocols: Difference between revisions

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==Silver Lab: Synthetic Biology==
=Nano Synth Bio=
*Note: These are protocols that I used and/or developed as a postdoctoral fellow in Pam Silver's lab.
==General Molecular Biology==
*[[Silver: BB Strategy | Strategy for Making Parts]]
[http://www.mrw.interscience.wiley.com/emrw/9780471142720/cp/cpmb/toc Current Protocols in Molecular Biology]
*[[Silver: Commonly Used Plasmids | Commonly Used Plasmids]]
*[[Silver: Commonly Used Primers | Commonly Used Primers]]
*[[Silver: PCR | PCR]]
*[[Silver: Oligonucleotide Inserts | Oligonucleotide Inserts]]
*[[Silver: Overlapping Oligonucleotide Inserts | Overlapping Oligonucleotide Inserts]]
*[[Silver: Site-Directed Mutagenesis | Site-Directed Mutagenesis]]
*[[Silver: Restriction Digest | Restriction Digest]]
*[[Silver: Ligation | Ligation]]
*[[Silver: Quick & Dirty Digest & Ligation | Quick & Dirty Digest & Ligation]]
*[[Silver: Bacterial Transformation | Bacterial Transformation]]
*[[Silver: Plasmid Verification | Plasmid Verification]]
*[[Silver: Integration into yeast | Integration into Yeast]]
*[[Silver: Verifying Genomic Integration | Verifying Genomic Integration]]


==New Stuff==
==Lab Manual==
*[[NanoBio: Designing Parts or Genes| Designing Parts or Genes ]]
*[[NanoBio: Notebook| Keeping a Laboratory Notebook]]
 
==Sub-cloning ==
*[[Arking:JCATutorialIntro7| Introduction to Cloning]] A nice introduction to cloning, written by Chris Anderson
*[[Arking:JCATutorialIntro1| Introduction to BioBricks]] A nice introduction to BioBrick cloning, written by Chris Anderson
*[[NanoBio: Installing Vector NTI| Installing Vector NTI]] How to install Vector NTI, a free sequence editor
*[[NanoBio: Biobrick/fusion Strategy | BioBrick & Biofusion Strategies for Making Parts]] An overview of how to make & combine parts.
*[[Synthetic_Biology:BioBricks/3A_assembly| 3 Antibiotic Assembly]]
*[[NanoBio: Commonly Used Plasmids | Commonly Used Plasmids]]
*[[NanoBio: Commonly Used Primers | Commonly Used Primers]]
*[[NanoBio: PCR | PCR]]
*[[NanoBio: Golden Gate Cloning | Golden Gate Cloning]]
*[[NanoBio: Oligonucleotide Inserts | Oligonucleotide Inserts]]
*[[NanoBio: Overlapping Oligonucleotide Inserts | Overlapping Oligonucleotide Inserts]]
*[[NanoBio: Site-Directed Mutagenesis | Site-Directed Mutagenesis]]
*[[NanoBio: Restriction Digest | Restriction Digest]]
*[[NanoBio: Ligation | Ligation]]
*[[NanoBio: Bacterial Transformation | Bacterial Transformation of Chemically Competent Cells]]
*[[NanoBio: Prep for Electrocompetent cells | Prep for Electrocompetent cells]]
*[[NanoBio: Bacterial Transformation using Electroporation | Bacterial Transformation using Electroporation]]
*[[NanoBio: Plasmid Verification | Plasmid Verification]]
*[[NanoBio: Archiving Your Plasmid in the Strain Collection | Archiving Your Plasmid in the Strain Collection]]
*[[NanoBio: AgaroseGels | Agarose Gels]]
 
==Protein Expression & Purification ==
*[[NanoBio: Bacterial Hosts for Overexpression|Bacterial Hosts for Overexpression]]
*[[NanoBio: Test Induction & Purification|Test Induction & Purification]]
*[[NanoBio: Protein Purification|Protein Purification]]
*[[NanoBio: Isolation of Periplasmic Fraction|Isolation of Periplasmic Fraction]]
*[[NanoBio: Protein Gels|Protein Gels]]
*[[NanoBio: Cell Surface Binding Assay|Cell Surface Binding Assay]]
 
==Gene deletion with λ-red ==
*[[NanoBio: Overview and Materials for λ-red|Overview and Materials for λ-red]]
*[[NanoBio: Primer Design|Primer Design]]
*[[NanoBio: Protocol for gene knockout|Protcol for gene knockout]]
*[[NanoBio: Knockout/in|Knockout/in]]
 
==Genomic Integration into S. cerevisiae==
*[[NanoBio: Integration into yeast | Integration into Yeast]]
*[[NanoBio: Verifying Genomic Integration | Verifying Genomic Integration]]
 
==Lipid Bilayers==
*[[NanoBio: Making Unilamellar Vesicles| Making Unilamellar Vesicles]]
*[[NanoBio: Preparation of Supported Bilayer| Preparation of Supported Bilayers]]
*[[NanoBio: Cleaning SiO2 surfaces| Cleaning SiO2 surfaces]]
 
==Care of Synechocystis sp 6803==
*[[NanoBio: Liquid & Solid Growth Media| Liquid & Solid Growth Media]]
*[http://openwetware.org/wiki/IGEM:Harvard/2006/Cyanobacteria/Protocols Guidelines from 2006 Harvard iGEM team]
 
==Primers==
* Biobricks & Biofusion Sub-cloning
*[[NanoBio: Synechocystis| Synechocystis sp. 6803]]
*[http://microbewiki.kenyon.edu/index.php/Shewanella_oneidensis Shewanella oneidensis MR-1]
*[[NanoBio: Guidelines|Guidelines on Talking or Writing about your project]]
*[[NanoBio: Microbial Respiration| Microbial Respiration]]
 
== Recipes ==
* [[NanoBio: LB plates | LB media & plates]]
* [[NanoBio: Antibiotics | Antibiotics]]
* [[NanoBio: TSB media | TSB media]]
* [[NanoBio: BG-11 media | BG-11 media]]
* [[NanoBio: BG-11 plates| BG-11 plates]]
* [[NanoBio: Supplemented M9 Media| Supplemented M9 Media]]
* [[NanoBio: Stock Solutions | Stock Solutions]]
 
==Safety & Waste Disposal==
*[[NanoBio: Safety & Waste Disposal | Safety & Waste Disposal]]
*[[NanoBio: Safety & Waste Disposal | Safety & Waste Disposal]]

Latest revision as of 16:12, 1 May 2013

Nano Synth Bio

General Molecular Biology

Current Protocols in Molecular Biology

Lab Manual

Sub-cloning

Protein Expression & Purification

Gene deletion with λ-red

Genomic Integration into S. cerevisiae

Lipid Bilayers

Care of Synechocystis sp 6803

Primers

Recipes

Safety & Waste Disposal

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