NanoBio:Protocols

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(Sub-cloning using Biobricks or Biofusion Vectors)
Current revision (19:12, 1 May 2013) (view source)
(Lab Practices)
 
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=Nano Synth Bio=
=Nano Synth Bio=
==General Molecular Biology==
==General Molecular Biology==
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[http://www.mrw.interscience.wiley.com/cp/cpmb/cpmb_contents_fs.html Current Protocols in Molecular Biology]
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[http://www.mrw.interscience.wiley.com/emrw/9780471142720/cp/cpmb/toc Current Protocols in Molecular Biology]
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==Lab Manual==
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*[[NanoBio: Notebook| Keeping a Laboratory Notebook]]
==Sub-cloning ==
==Sub-cloning ==
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*[[NanoBio: Biobrick/fusion Strategy | BioBrick & Biofusion Strategies for Making Parts]]
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*[[Arking:JCATutorialIntro7| Introduction to Cloning]] A nice introduction to cloning, written by Chris Anderson
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*[[NanoBio: Designing Parts or Genes| Designing Parts or Genes ]]
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*[[Arking:JCATutorialIntro1| Introduction to BioBricks]] A nice introduction to BioBrick cloning, written by Chris Anderson
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*[[NanoBio: Installing Vector NTI| Installing Vector NTI]] How to install Vector NTI, a free sequence editor
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*[[NanoBio: Biobrick/fusion Strategy | BioBrick & Biofusion Strategies for Making Parts]] An overview of how to make & combine parts.
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*[[Synthetic_Biology:BioBricks/3A_assembly| 3 Antibiotic Assembly]]
*[[NanoBio: Commonly Used Plasmids | Commonly Used Plasmids]]
*[[NanoBio: Commonly Used Plasmids | Commonly Used Plasmids]]
*[[NanoBio: Commonly Used Primers | Commonly Used Primers]]
*[[NanoBio: Commonly Used Primers | Commonly Used Primers]]
*[[NanoBio: PCR | PCR]]
*[[NanoBio: PCR | PCR]]
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*[[NanoBio: Golden Gate Cloning | Golden Gate Cloning]]
*[[NanoBio: Oligonucleotide Inserts | Oligonucleotide Inserts]]
*[[NanoBio: Oligonucleotide Inserts | Oligonucleotide Inserts]]
*[[NanoBio: Overlapping Oligonucleotide Inserts | Overlapping Oligonucleotide Inserts]]
*[[NanoBio: Overlapping Oligonucleotide Inserts | Overlapping Oligonucleotide Inserts]]
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*[[NanoBio: Restriction Digest | Restriction Digest]]
*[[NanoBio: Restriction Digest | Restriction Digest]]
*[[NanoBio: Ligation | Ligation]]
*[[NanoBio: Ligation | Ligation]]
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*[[NanoBio: 3 Antibiotic Assembly| 3 Antibiotic Assembly]]
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*[[NanoBio: Bacterial Transformation | Bacterial Transformation of Chemically Competent Cells]]
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*[[NanoBio: Bacterial Transformation | Bacterial Transformation]]
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*[[NanoBio: Prep for Electrocompetent cells | Prep for Electrocompetent cells]]
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*[[NanoBio: Bacterial Transformation using Electroporation | Bacterial Transformation using Electroporation]]
*[[NanoBio: Plasmid Verification | Plasmid Verification]]
*[[NanoBio: Plasmid Verification | Plasmid Verification]]
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*Note: Many of these are protocols that I used and/or developed as a postdoctoral fellow in Pam Silver's lab.
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*[[NanoBio: Archiving Your Plasmid in the Strain Collection | Archiving Your Plasmid in the Strain Collection]]
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*[[NanoBio: AgaroseGels | Agarose Gels]]
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==Protein Expression & Purification ==
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*[[NanoBio: Bacterial Hosts for Overexpression|Bacterial Hosts for Overexpression]]
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*[[NanoBio: Test Induction & Purification|Test Induction & Purification]]
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*[[NanoBio: Protein Purification|Protein Purification]]
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*[[NanoBio: Isolation of Periplasmic Fraction|Isolation of Periplasmic Fraction]]
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*[[NanoBio: Protein Gels|Protein Gels]]
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*[[NanoBio: Cell Surface Binding Assay|Cell Surface Binding Assay]]
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==Gene deletion with λ-red ==
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*[[NanoBio: Overview and Materials for λ-red|Overview and Materials for λ-red]]
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*[[NanoBio: Primer Design|Primer Design]]
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*[[NanoBio: Protocol for gene knockout|Protcol for gene knockout]]
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*[[NanoBio: Knockout/in|Knockout/in]]
==Genomic Integration into S. cerevisiae==
==Genomic Integration into S. cerevisiae==
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*[[Silver: Integration into yeast | Integration into Yeast]]
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*[[NanoBio: Integration into yeast | Integration into Yeast]]
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*[[Silver: Verifying Genomic Integration | Verifying Genomic Integration]]
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*[[NanoBio: Verifying Genomic Integration | Verifying Genomic Integration]]
==Lipid Bilayers==
==Lipid Bilayers==
*[[NanoBio: Making Unilamellar Vesicles| Making Unilamellar Vesicles]]
*[[NanoBio: Making Unilamellar Vesicles| Making Unilamellar Vesicles]]
*[[NanoBio: Preparation of Supported Bilayer| Preparation of Supported Bilayers]]
*[[NanoBio: Preparation of Supported Bilayer| Preparation of Supported Bilayers]]
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*[[NanoBio: Cleaning SiO2 surfaces| Cleaning SiO2 surfaces]]
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==Care of Synechocystis sp 6803==
==Care of Synechocystis sp 6803==
*[[NanoBio: Liquid & Solid Growth Media| Liquid & Solid Growth Media]]
*[[NanoBio: Liquid & Solid Growth Media| Liquid & Solid Growth Media]]
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* [[NanoBio: BG-11 media | BG-11 media]]
* [[NanoBio: BG-11 media | BG-11 media]]
* [[NanoBio: BG-11 plates| BG-11 plates]]
* [[NanoBio: BG-11 plates| BG-11 plates]]
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* [[NanoBio: Supplemented M9 Media| Supplemented M9 Media]]
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* [[NanoBio: Stock Solutions | Stock Solutions]]
==Safety & Waste Disposal==
==Safety & Waste Disposal==
*[[NanoBio: Safety & Waste Disposal | Safety & Waste Disposal]]
*[[NanoBio: Safety & Waste Disposal | Safety & Waste Disposal]]

Current revision

Contents

Nano Synth Bio

General Molecular Biology

Current Protocols in Molecular Biology

Lab Manual

Sub-cloning

Protein Expression & Purification

Gene deletion with λ-red

Genomic Integration into S. cerevisiae

Lipid Bilayers

Care of Synechocystis sp 6803

Primers

Recipes

Safety & Waste Disposal

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