NanoBio:Protocols: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
| Line 32: | Line 32: | ||
* Biobricks & Biofusion Sub-cloning | * Biobricks & Biofusion Sub-cloning | ||
*[[NanoBio: Synechocystis| Synechocystis sp. 6803]] | *[[NanoBio: Synechocystis| Synechocystis sp. 6803]] | ||
*[http://microbewiki.kenyon.edu/index.php/Shewanella_oneidensis Shewanella oneidensis MR-1] | |||
*[[NanoBio: Guidelines|Guidelines on Talking or Writing about your project]] | *[[NanoBio: Guidelines|Guidelines on Talking or Writing about your project]] | ||
*[[NanoBio: Microbial Respiration| Microbial Respiration]] | *[[NanoBio: Microbial Respiration| Microbial Respiration]] | ||
Revision as of 11:28, 4 December 2007
Nano Synth Bio
General Molecular Biology
Current Protocols in Molecular Biology
Sub-cloning using Biobricks or Biofusion Vectors
- Note: Many of these are protocols that I used and/or developed as a postdoctoral fellow in Pam Silver's lab.
- Strategy for Making Parts
- Designing Parts or Genes
- Commonly Used Plasmids
- Commonly Used Primers
- PCR
- Oligonucleotide Inserts
- Overlapping Oligonucleotide Inserts
- Site-Directed Mutagenesis
- Restriction Digest
- Ligation
- Quick & Dirty Digest & Ligation
- Bacterial Transformation
- Plasmid Verification
Genomic Integration into S. cerevisiae
Lipid Bilayers
Care of Synechocystis sp 6803
Primers
- Biobricks & Biofusion Sub-cloning
- Synechocystis sp. 6803
- Shewanella oneidensis MR-1
- Guidelines on Talking or Writing about your project
- Microbial Respiration
