NanoBio:Protocols: Difference between revisions
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*[[Silver: Bacterial Transformation | Bacterial Transformation]] | *[[Silver: Bacterial Transformation | Bacterial Transformation]] | ||
*[[Silver: Plasmid Verification | Plasmid Verification]] | *[[Silver: Plasmid Verification | Plasmid Verification]] | ||
*[[NanoBio: Ligation | Ligation]] | |||
*[[NanoBio: 3 Antibiotic Assembly| 3 Antibiotic Assembly]] | |||
*[[NanoBio: Bacterial Transformation | Bacterial Transformation]] | |||
*[[NanoBio: Plasmid Verification | Plasmid Verification]] | |||
==Genomic Integration into S. cerevisiae== | ==Genomic Integration into S. cerevisiae== | ||
Revision as of 18:39, 29 January 2008
Nano Synth Bio
General Molecular Biology
Current Protocols in Molecular Biology
Sub-cloning using Biobricks or Biofusion Vectors
- Note: Many of these are protocols that I used and/or developed as a postdoctoral fellow in Pam Silver's lab.
- Strategy for Making Parts
- Designing Parts or Genes
- Commonly Used Plasmids
- Commonly Used Primers
- PCR
- Oligonucleotide Inserts
- Overlapping Oligonucleotide Inserts
- Site-Directed Mutagenesis
- Restriction Digest
- Ligation
- Quick & Dirty Digest & Ligation
- Bacterial Transformation
- Plasmid Verification
- Ligation
- 3 Antibiotic Assembly
- Bacterial Transformation
- Plasmid Verification
Genomic Integration into S. cerevisiae
Lipid Bilayers
Care of Synechocystis sp 6803
Primers
- Biobricks & Biofusion Sub-cloning
- Synechocystis sp. 6803
- Shewanella oneidensis MR-1
- Guidelines on Talking or Writing about your project
- Microbial Respiration
