NanoBio:Protocols: Difference between revisions
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=Nano Synth Bio= | =Nano Synth Bio= | ||
==General Molecular Biology== | ==General Molecular Biology== | ||
[http://www.mrw.interscience.wiley.com/cp/cpmb/ | [http://www.mrw.interscience.wiley.com/emrw/9780471142720/cp/cpmb/toc Current Protocols in Molecular Biology] | ||
==Sub-cloning == | ==Sub-cloning == | ||
Revision as of 15:14, 5 June 2008
Nano Synth Bio
General Molecular Biology
Current Protocols in Molecular Biology
Sub-cloning
- Introduction to Cloning A nice introduction to cloning, written by Chris Anderson
- Introduction to BioBricks A nice introduction to BioBrick cloning, written by Chris Anderson
- BioBrick & Biofusion Strategies for Making Parts
- Designing Parts or Genes
- Commonly Used Plasmids
- Commonly Used Primers
- PCR
- Oligonucleotide Inserts
- Overlapping Oligonucleotide Inserts
- Site-Directed Mutagenesis
- Restriction Digest
- Ligation
- 3 Antibiotic Assembly
- Bacterial Transformation
- Plasmid Verification
- Note: Many of these are protocols that I used and/or developed as a postdoctoral fellow in Pam Silver's lab.
Genomic Integration into S. cerevisiae
Lipid Bilayers
Care of Synechocystis sp 6803
Primers
- Biobricks & Biofusion Sub-cloning
- Synechocystis sp. 6803
- Shewanella oneidensis MR-1
- Guidelines on Talking or Writing about your project
- Microbial Respiration
