NanoBio:Protocols: Difference between revisions
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==General Molecular Biology== | ==General Molecular Biology== | ||
[http://www.mrw.interscience.wiley.com/emrw/9780471142720/cp/cpmb/toc Current Protocols in Molecular Biology] | [http://www.mrw.interscience.wiley.com/emrw/9780471142720/cp/cpmb/toc Current Protocols in Molecular Biology] | ||
==Lab Practices== | |||
*[[NanoBio: Notebook| Keeping a Laboratory Notebook]] | |||
==Sub-cloning == | ==Sub-cloning == |
Revision as of 10:42, 19 June 2009
Nano Synth Bio
General Molecular Biology
Current Protocols in Molecular Biology
Lab Practices
Sub-cloning
- Introduction to Cloning A nice introduction to cloning, written by Chris Anderson
- Introduction to BioBricks A nice introduction to BioBrick cloning, written by Chris Anderson
- Installing Vector NTI How to install Vector NTI, a free sequence editor
- BioBrick & Biofusion Strategies for Making Parts An overview of how to make & combine parts.
- 3 Antibiotic Assembly
- Commonly Used Plasmids
- Commonly Used Primers
- PCR
- Oligonucleotide Inserts
- Overlapping Oligonucleotide Inserts
- Site-Directed Mutagenesis
- Restriction Digest
- Ligation
- Bacterial Transformation of Chemically Competent Cells
- Prep for Electrocompetent cells
- Bacterial Transformation using Electroporation
- Plasmid Verification
- Agarose Gels
Protein Expression & Purification
- Bacterial Hosts for Overexpression
- Test Induction & Purification
- Protein Purification
- Protein Gels
- Cell Surface Binding Assay
Gene deletion with λ-red
Genomic Integration into S. cerevisiae
Lipid Bilayers
Care of Synechocystis sp 6803
Primers
- Biobricks & Biofusion Sub-cloning
- Synechocystis sp. 6803
- Shewanella oneidensis MR-1
- Guidelines on Talking or Writing about your project
- Microbial Respiration