NanoBio:Protocols: Difference between revisions
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*[[NanoBio: Primer Design|Primer Design]] | *[[NanoBio: Primer Design|Primer Design]] | ||
*[[NanoBio: Protocol for gene knockout|Protcol for gene knockout]] | *[[NanoBio: Protocol for gene knockout|Protcol for gene knockout]] | ||
*[[NanoBio: Knockout/in|Knockout/in]] | |||
==Genomic Integration into S. cerevisiae== | ==Genomic Integration into S. cerevisiae== | ||
Revision as of 12:42, 10 August 2009
Nano Synth Bio
General Molecular Biology
Current Protocols in Molecular Biology
Lab Practices
Sub-cloning
- Introduction to Cloning A nice introduction to cloning, written by Chris Anderson
- Introduction to BioBricks A nice introduction to BioBrick cloning, written by Chris Anderson
- Installing Vector NTI How to install Vector NTI, a free sequence editor
- BioBrick & Biofusion Strategies for Making Parts An overview of how to make & combine parts.
- 3 Antibiotic Assembly
- Commonly Used Plasmids
- Commonly Used Primers
- PCR
- Oligonucleotide Inserts
- Overlapping Oligonucleotide Inserts
- Site-Directed Mutagenesis
- Restriction Digest
- Ligation
- Bacterial Transformation of Chemically Competent Cells
- Prep for Electrocompetent cells
- Bacterial Transformation using Electroporation
- Plasmid Verification
- Agarose Gels
Protein Expression & Purification
- Bacterial Hosts for Overexpression
- Test Induction & Purification
- Protein Purification
- Isolation of Periplasmic Fraction
- Protein Gels
- Cell Surface Binding Assay
Gene deletion with λ-red
Genomic Integration into S. cerevisiae
Lipid Bilayers
Care of Synechocystis sp 6803
Primers
- Biobricks & Biofusion Sub-cloning
- Synechocystis sp. 6803
- Shewanella oneidensis MR-1
- Guidelines on Talking or Writing about your project
- Microbial Respiration
