NanoBio:Protocols

Synthetic Biology

• Note: These are protocols that I used and/or developed as a postdoctoral fellow in Pam Silver's lab.
• Strategy for Making Parts
• Commonly Used Plasmids
• Commonly Used Primers
• PCR
• Oligonucleotide Inserts
• Overlapping Oligonucleotide Inserts
• Site-Directed Mutagenesis
• Restriction Digest
• Ligation
• Quick & Dirty Digest & Ligation
• Bacterial Transformation
• Plasmid Verification
• Integration into Yeast
• Verifying Genomic Integration