NanoBio:Protocols

Nano Synth Bio

General Molecular Biology

Current Protocols in Molecular Biology

Sub-cloning

• Introduction to Cloning A nice introduction to cloning, written by Chris Anderson
• Introduction to BioBricks A nice introduction to BioBrick cloning, written by Chris Anderson
• Installing Vector NTI How to install Vector NTI, a free sequence editor
• BioBrick & Biofusion Strategies for Making Parts
• Designing Parts or Genes
• Commonly Used Plasmids
• Commonly Used Primers
• PCR
• Oligonucleotide Inserts
• Overlapping Oligonucleotide Inserts
• Site-Directed Mutagenesis
• Restriction Digest
• Ligation
• 3 Antibiotic Assembly
• Bacterial Transformation
• Plasmid Verification

Protein Expression & Purification

• Bacterial Hosts for Overexpression
• Test Induction & Purification
• Protein Purification

Genomic Integration into S. cerevisiae

• Integration into Yeast
• Verifying Genomic Integration

Lipid Bilayers

• Making Unilamellar Vesicles
• Preparation of Supported Bilayers

Care of Synechocystis sp 6803

• Liquid & Solid Growth Media
• Guidelines from 2006 Harvard iGEM team

Primers

• Biobricks & Biofusion Sub-cloning
• Synechocystis sp. 6803
• Shewanella oneidensis MR-1
• Guidelines on Talking or Writing about your project
• Microbial Respiration

Recipes

• LB media & plates
• Antibiotics
• TSB media
• BG-11 media
• BG-11 plates

Safety & Waste Disposal

• Safety & Waste Disposal