NanoBio: Archiving Your Plasmid in the Strain Collection: Difference between revisions

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(New page: == Archiving the New Part == * All new strains should be listed in our spreadsheet of strains and stored in the Molecular Foundry strain collection. It is also helpful to keep parts that y...)
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Revision as of 13:04, 21 March 2013

Archiving the New Part

  • All new strains should be listed in our spreadsheet of strains and stored in the Molecular Foundry strain collection. It is also helpful to keep parts that you have made in your personal strain collection.
  • It is recommended that you also add new plasmids to the plasmid collection.

Adding a Strain to the Strain Collection

  1. Open Plasmid_Cell Stock Registry in Excel.
  2. Give your plasmid a name.
    • Plasmids are names Lxxxx, where L is a letter and x is a number.
    • The letter indicates the function of the part. Currently
      • C=Binding Proteins
      • D=Delta (Knockout strains)
      • E=Reporter Proteins
      • G=Genetic Recombination Parts

H=Thermo Stability

      • I=Electron Transfer Proteins
      • J=Transporters
      • K=RBS's, translational parts
      • L=terminators, transcriptional modifiers
      • P=Promoters, binding sites
      • S=Structural proteins
      • T=tags
      • Z=Combination Parts

Plasmid Collection

  • Place 5 uL mini-prepped plasmid DNA in a labeled eppendorf tube in the appropriate plasmid collection freezer box in the -20 freezer in Rm 5204.
  • For the strain collection, grow a bacterial culture either to mid-exponential phase or overnight. Mix equal volumes 50% glycerol and culture in an autoclaved 2 mL screw-top tube. Store at -80.

these are the blue labeled boxes on the Common Stocks shelf in the -80C in Rm 5204.

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