NanoBio: BG-11 plates

From OpenWetWare

Revision as of 12:49, 28 September 2007 by CarolineAjo-Franklin (Talk | contribs)
(diff) ←Older revision | Current revision (diff) | Newer revision→ (diff)
Jump to: navigation, search

BG-11 plates

  • For 1 L, or ~2 sleeves of plates, place in a 2L flask:
    • 10 mL 1 M TES pH 8.2 (Sigma catalog #)
    • 3 g sodium thiosulfate (Sigma, catalog #)
    • 15 g Bacto-agar (catalog #)
    • 20 mL 50x BG-11 media (Sigma catalog #)
    • 980 mL distilled water
    • a stirring bar
  • Autoclave at 121C for 20 minutes on the liquid cycle.
Personal tools