NanoBio: Commonly Used Primers: Difference between revisions
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(New page: == Commonly Used Primers == * Sequencing primers for Biobrick & Biofusion vectors: ** pSB1A3, pSB1AC3, pSB1AK3, pSB1AT3 forward primer: 5' AAATAGGCGTATCACGAGGC 3' ** pSB1A3, pSB1AC3, pSB1...) |
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* Sequencing primers for other vectors | * Sequencing primers for other vectors | ||
pET vectors 5' atgcgtccggcgtaga | **pET vectors 5' atgcgtccggcgtaga | ||
T7 promoter 5' taatacgactcactataggg | **T7 promoter 5' taatacgactcactataggg | ||
T7 terminator 5' gctagttattgctcagcgg | **T7 terminator 5' gctagttattgctcagcgg | ||
*'''[[User:CarolineAjo-Franklin|CAjoF]] 20:27, 29 January 2008 (CST)''': | *'''[[User:CarolineAjo-Franklin|CAjoF]] 20:27, 29 January 2008 (CST)''': | ||
Revision as of 19:29, 29 January 2008
Commonly Used Primers
- Sequencing primers for Biobrick & Biofusion vectors:
- pSB1A3, pSB1AC3, pSB1AK3, pSB1AT3 forward primer: 5' AAATAGGCGTATCACGAGGC 3'
- pSB1A3, pSB1AC3, pSB1AK3, pSB1AT3 reverse primer: 5' GAGTCAGTGAGCGAGGAAGC 3'
- V0100/V0120 forward sequencing primer: 5' GGGTTTTCCCAGTCACGACG 3'
- V0100/V0120 reverse sequencing primer: 5' TGTGGAATTGTGAGCGGATAACA 3'
- V0002 forward sequencing primer: 5' tttctggaattcgcggcc 3'
- V0002 reverse sequencing primer: 5' gccggactgcagcggcc 3'
- Primers that sequence regions between the Biobrick ends
- upstream of a Kozak region (K0001): 5' TCTAGTCTCCATGGTGGCGG 3'
- downstream of a Kozak region (K0001): 5' CCGCCACCATGGAGACTAGA 3'
- upstream of the ADH1 terminator (L0100): 5' CCTGAGAAAGCAACCTGACCTACAG 3'
- downstream of midpoint btwn yeast-codon optimized YFP/mCherryx2 (Z0035 or Z0037): 5' GGTACCGCAACTAGAGCAACTAGC 3'
- upstream of midpoint btwn yeast-codon optimized YFP/mCherryx2(Z0035 or Z0037): 5' GCTAGTTGCTCTAGTTGCGGTACC 3'
- Sequencing primers for other vectors
- pET vectors 5' atgcgtccggcgtaga
- T7 promoter 5' taatacgactcactataggg
- T7 terminator 5' gctagttattgctcagcgg
- CAjoF 20:27, 29 January 2008 (CST):
