NanoBio: Primer Design

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Revision as of 11:52, 9 June 2009 by Heather M. Jensen (talk | contribs) (New page: ==Primer Design== You will need 4 primers per knockout that you design. *2 will be for the PCR to clone out the antibiotic resistance. These are 70 bases long. *The other 2 are for verify...)
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Primer Design

You will need 4 primers per knockout that you design.

  • 2 will be for the PCR to clone out the antibiotic resistance. These are 70 bases long.
  • The other 2 are for verifying knockouts with colony PCR. These are 25 bases long.


Primer Set 1

This is the trickier set. Be careful! I will use NapC from E. coli as an example here.


Primer Set 2

This is the easier set! Just copy and paste the fist 25 bases of each of the above primers, and you're done!


--Heather M. Jensen 14:52, 9 June 2009 (EDT)

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