NanoBio: Supplemented M9 Media: Difference between revisions
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(New page: This recipe makes 1L of media supplemented with *xxx% of selected carbon source *1 mM thiamine hydrochloride *0.2% casamino acids Combine all solutions using sterile technique: *200 mL 5...) |
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=M9 media= | |||
This recipe makes 1L of media supplemented with | This recipe makes 1L of media supplemented with | ||
*xxx% of selected carbon source | *xxx% of selected carbon source | ||
*1 mM thiamine hydrochloride | *1 mM thiamine hydrochloride | ||
*0.2% casamino acids | *0.2% casamino acids | ||
Combine all solutions using sterile technique: | Combine all solutions using sterile technique: | ||
Revision as of 14:56, 29 July 2008
M9 media
This recipe makes 1L of media supplemented with
- xxx% of selected carbon source
- 1 mM thiamine hydrochloride
- 0.2% casamino acids
Combine all solutions using sterile technique:
- 200 mL 5x M9 salts
- 30 mL of 10mg/mL thaimine hydrochlorite
- Dissolve 10mg per mL of H2O
- Filter-sterilize using a 0.22μm filter
- Light-sensitive. Store covered in foil
- 20 mL of 20% carbon source
- 20 mL of 10% casamino acids
- 20 mL of 0.1 M MgSO4
- 1 mL of 0.1 M CaCl2
- deionized water to 1L (709.8mL)
Store at 4°C.
Note: Adding Fe increases cell density.
