NanoBio: Supplemented M9 Media: Difference between revisions
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*'''[[User:Heather M. Jensen|Heather M. Jensen]] 19:52, 11 June 2008 (CST)''': | *'''[[User:Heather M. Jensen|Heather M. Jensen]] 19:52, 11 June 2008 (CST)''': | ||
Return to [[NanoBio:Media|Recipes]]. | |||
Return to [[NanoBio:Protocols|Protocols]]. | Return to [[NanoBio:Protocols|Protocols]]. |
Revision as of 15:01, 29 July 2008
M9 media
This recipe makes 1L of media supplemented with
- 0.4% of selected carbon source
- Examples of carbon source: glucose, lactate, glycerol, succinate, acetate, fumarate
- 1 mM thiamine hydrochloride
- 0.2% casamino acids
Combine all solutions using sterile technique:
- 200 mL 5x M9 salts
- 30 mL of 10mg/mL thaimine hydrochlorite
- Dissolve 10mg per mL of H2O
- Filter-sterilize using a 0.22μm filter
- Light-sensitive. Store covered in foil
- 20 mL of 20% carbon source
- 20 mL of 10% casamino acids
- 20 mL of 0.1 M MgSO4
- 1 mL of 0.1 M CaCl2
- deionized water to 1L (709.8mL)
Store at 4°C.
Note: Adding Fe increases cell density. For example, FeSO4·7H2O at 0.01 mM
- Heather M. Jensen 19:52, 11 June 2008 (CST):