NanoBio: Supplemented M9 Media: Difference between revisions
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(New page: This recipe makes 1L of media supplemented with *xxx% of selected carbon source *1 mM thiamine hydrochloride *0.2% casamino acids Combine all solutions using sterile technique: *200 mL 5...) |
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=M9 media= | |||
This recipe makes 1L of media supplemented with | This recipe makes 1L of media supplemented with | ||
* | *0.4% of selected carbon source | ||
**Examples of carbon source: glucose, lactate, glycerol, succinate, acetate, fumarate | |||
*1 mM thiamine hydrochloride | *1 mM thiamine hydrochloride | ||
*0.2% casamino acids | *0.2% casamino acids | ||
Combine all solutions using sterile technique: | Combine all solutions using sterile technique: | ||
| Line 18: | Line 22: | ||
Store at 4°C. | Store at 4°C. | ||
Note: Adding Fe increases cell density. | Note: Adding Fe increases cell density. For example, FeSO<sub>4</sub>·7H<sub>2</sub>O at 0.01 mM | ||
*'''[[User:Heather M. Jensen|Heather M. Jensen]] 19:52, 11 June 2008 (CST)''': | |||
Return to [[NanoBio:Protocols|Protocols]]. | |||
Latest revision as of 15:02, 29 July 2008
M9 media
This recipe makes 1L of media supplemented with
- 0.4% of selected carbon source
- Examples of carbon source: glucose, lactate, glycerol, succinate, acetate, fumarate
- 1 mM thiamine hydrochloride
- 0.2% casamino acids
Combine all solutions using sterile technique:
- 200 mL 5x M9 salts
- 30 mL of 10mg/mL thaimine hydrochlorite
- Dissolve 10mg per mL of H2O
- Filter-sterilize using a 0.22μm filter
- Light-sensitive. Store covered in foil
- 20 mL of 20% carbon source
- 20 mL of 10% casamino acids
- 20 mL of 0.1 M MgSO4
- 1 mL of 0.1 M CaCl2
- deionized water to 1L (709.8mL)
Store at 4°C.
Note: Adding Fe increases cell density. For example, FeSO4·7H2O at 0.01 mM
- Heather M. Jensen 19:52, 11 June 2008 (CST):
Return to Protocols.
