NanoBio: Supplemented M9 Media
From OpenWetWare
This recipe makes 1L of media supplemented with
- xxx% of selected carbon source
- 1 mM thiamine hydrochloride
- 0.2% casamino acids
Combine all solutions using sterile technique:
- 200 mL 5x M9 salts
- 30 mL of 10mg/mL thaimine hydrochlorite
- Dissolve 10mg per mL of H2O
- Filter-sterilize using a 0.22μm filter
- Light-sensitive. Store covered in foil
- 20 mL of 20% carbon source
- 20 mL of 10% casamino acids
- 20 mL of 0.1 M MgSO4
- 1 mL of 0.1 M CaCl2
- deionized water to 1L (709.8mL)
Store at 4°C.
Note: Adding Fe increases cell density.