NanoBio: Supplemented M9 Media

M9 media

This recipe makes 1L of media supplemented with

• 0.4% of selected carbon source
  • Examples of carbon source: glucose, lactate, glycerol, succinate, acetate, fumarate
• 1 mM thiamine hydrochloride
• 0.2% casamino acids

Combine all solutions using sterile technique:

• 200 mL 5x M9 salts
• 30 mL of 10mg/mL thaimine hydrochlorite
  1. Dissolve 10mg per mL of H₂O
  2. Filter-sterilize using a 0.22μm filter
  3. Light-sensitive. Store covered in foil
• 20 mL of 20% carbon source
• 20 mL of 10% casamino acids
• 20 mL of 0.1 M MgSO₄
• 1 mL of 0.1 M CaCl₂
• deionized water to 1L (709.8mL)

Store at 4°C.

Note: Adding Fe increases cell density. For example, FeSO₄·7H₂O at 0.01 mM

• Heather M. Jensen 19:52, 11 June 2008 (CST):

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