NanoBio: Supplemented M9 Media

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M9 media

This recipe makes 1L of media supplemented with

  • 0.4% of selected carbon source
    • Examples of carbon source: glucose, lactate, glycerol, succinate, acetate, fumarate
  • 1 mM thiamine hydrochloride
  • 0.2% casamino acids


Combine all solutions using sterile technique:

  • 200 mL 5x M9 salts
  • 30 mL of 10mg/mL thaimine hydrochlorite
    1. Dissolve 10mg per mL of H2O
    2. Filter-sterilize using a 0.22μm filter
    3. Light-sensitive. Store covered in foil
  • 20 mL of 20% carbon source
  • 20 mL of 10% casamino acids
  • 20 mL of 0.1 M MgSO4
  • 1 mL of 0.1 M CaCl2
  • deionized water to 1L (709.8mL)

Store at 4°C.

Note: Adding Fe increases cell density. For example, FeSO4·7H2O at 0.01 mM


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