Nelson:Protocols: Difference between revisions
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*[[DNA_ligation]]<br> | *[[DNA_ligation]]<br> | ||
*Quickchange Mutagenesis [[Knight:Site-directed_mutagenesis/Single_site]]<br> | *Quickchange Mutagenesis [[Knight:Site-directed_mutagenesis/Single_site]]<br> | ||
* | *[http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/d3764pis.Par.0001.File.tmp/d3764pis.pdf preparing DEAE-cellulose ] | ||
Revision as of 09:29, 3 April 2009
Recipes
LB
M9 medium/minimal
TBE
SOB
SOC
10 X SDS-PAGE running buffer
50 X TAE buffer
Phosphate_buffer
Protocols
*Rubidium Chloride competent cells Wiese_Lab:Competent_Cell_Prep
*Calcium Chloride competent cells Kafatos:competent cells
*Qiagen Miniprep Minipreps
*DNA_Precipitation
*DNA_ligation
*Quickchange Mutagenesis Knight:Site-directed_mutagenesis/Single_site
*preparing DEAE-cellulose
