Nelson:Protocols

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(Protocols)
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  *[[DNA_ligation]]<br>
  *[[DNA_ligation]]<br>
  *Quickchange Mutagenesis [[Knight:Site-directed_mutagenesis/Single_site]]<br>
  *Quickchange Mutagenesis [[Knight:Site-directed_mutagenesis/Single_site]]<br>
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  *preparing DEAE-cellulose [http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/d3764pis.Par.0001.File.tmp/d3764pis.pdf]
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  *[http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/d3764pis.Par.0001.File.tmp/d3764pis.pdf preparing DEAE-cellulose ]

Revision as of 11:29, 3 April 2009

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Recipes

LB 
M9 medium/minimal
TBE
SOB
SOC
10 X SDS-PAGE running buffer
50 X TAE buffer
Phosphate_buffer

Protocols

*Rubidium Chloride competent cells Wiese_Lab:Competent_Cell_Prep
*Calcium Chloride competent cells Kafatos:competent cells
*Qiagen Miniprep Minipreps
*DNA_Precipitation
*DNA_ligation
*Quickchange Mutagenesis Knight:Site-directed_mutagenesis/Single_site
*preparing DEAE-cellulose
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