Nelson:Protocols

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== Protocols ==
== Protocols ==
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  *Rubidium Chloride competent cells [[Wiese_Lab:Competent_Cell_Prep]]<br>
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  Rubidium Chloride competent cells [[Wiese_Lab:Competent_Cell_Prep]]<br>
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  *Calcium Chloride competent cells [[Kafatos:competent cells]]<br>
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  Calcium Chloride competent cells [[Kafatos:competent cells]]<br>
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  *Qiagen Miniprep [[Minipreps]]<br>
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  Qiagen Miniprep [[Minipreps]]<br>
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  *[[DNA_Precipitation]]<br>
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  [[DNA_Precipitation]]<br>
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  *[[DNA_ligation]]<br>
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  [[DNA_ligation]]<br>
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  *Quickchange Mutagenesis [[Knight:Site-directed_mutagenesis/Single_site]]<br>
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  Quickchange Mutagenesis [[Knight:Site-directed_mutagenesis/Single_site]]<br>
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  *[http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/d3764pis.Par.0001.File.tmp/d3764pis.pdf preparing DEAE-cellulose ]
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  [http://www.sigmaaldrich.com/etc/medialib/docs/Sigma/Product_Information_Sheet/d3764pis.Par.0001.File.tmp/d3764pis.pdf preparing DEAE-cellulose ]

Revision as of 12:30, 3 April 2009

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Recipes

LB 
M9 medium/minimal
TBE
SOB
SOC
10 X SDS-PAGE running buffer
50 X TAE buffer
Phosphate_buffer

Protocols

Rubidium Chloride competent cells Wiese_Lab:Competent_Cell_Prep
Calcium Chloride competent cells Kafatos:competent cells
Qiagen Miniprep Minipreps
DNA_Precipitation
DNA_ligation
Quickchange Mutagenesis Knight:Site-directed_mutagenesis/Single_site
preparing DEAE-cellulose
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