Nelson:Protocols

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m (Protocols)
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  Rubidium Chloride competent cells [[Wiese_Lab:Competent_Cell_Prep]]<br>
  Rubidium Chloride competent cells [[Wiese_Lab:Competent_Cell_Prep]]<br>
  [http://ivaan.com/protocols/135.html Calcium Chloride competent cells ]<br>
  [http://ivaan.com/protocols/135.html Calcium Chloride competent cells ]<br>
 +
[http://ivaan.com/protocols/133.html transformation of chemically competent E. coli]<br>
  Qiagen Miniprep [[Minipreps]]<br>
  Qiagen Miniprep [[Minipreps]]<br>
  [[DNA_Precipitation]]<br>
  [[DNA_Precipitation]]<br>

Revision as of 08:29, 7 April 2009

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Recipes

LB 
M9 medium/minimal
TBE
SOB
SOC
10 X SDS-PAGE running buffer
50 X TAE buffer
Phosphate_buffer

Protocols

Rubidium Chloride competent cells Wiese_Lab:Competent_Cell_Prep
Calcium Chloride competent cells

transformation of chemically competent E. coli

Qiagen Miniprep Minipreps
DNA_Precipitation
DNA_ligation
Quickchange Mutagenesis Knight:Site-directed_mutagenesis/Single_site
preparing DEAE-cellulose
Personal tools