Nelson:To Do - Eric: Difference between revisions
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==The List== | ==The List== | ||
*dishes | |||
*prepare CaCl<sub>2</sub> competent Rosetta pLys cells | |||
*transform RNAp into freshly made competent cells | |||
*monitor cells in shaker and move to cold room when OD600 reaches 0.5 | |||
*start O/N culture of XL1-blue cells | |||
*aliquot and freeze RNaseH1 protein | |||
Revision as of 07:01, 8 April 2009
The List
- dishes
- prepare CaCl2 competent Rosetta pLys cells
- transform RNAp into freshly made competent cells
- monitor cells in shaker and move to cold room when OD600 reaches 0.5
- start O/N culture of XL1-blue cells
- aliquot and freeze RNaseH1 protein
