Nelson:To Do - Eric: Difference between revisions

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==The List==
==The List==


 
*do ATPase assay on mitochondrial helicase
*pour 2 SDS-PAGE gels
*prepare DEAE cellulose according to instructions (look for it on protocols page)
*make 1 L of 1 M Tris-HCl, pH 8.0
*6L of LB and autoclave
*transform polg-a into Rosetta cells
*make tet-LB plates <br>
*streak Xl1-blue onto tet-LB plate <br>
*prepare top-agar <br>
*monitor cells (your mutant) in shaker and turn down to 15 deg. C when OD reaches about 0.5<br>

Latest revision as of 08:57, 21 April 2009

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The List

  • do ATPase assay on mitochondrial helicase
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