Nelson:To Do - Eric: Difference between revisions

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==The List==
==The List==
*dishes
 
*prepare CaCl<sub>2</sub> competent Rosetta pLys cells
*do ATPase assay on mitochondrial helicase
*make LB-AMP/Chloro plates
*transform RNAp into freshly made competent cells
*make 4 M NaCl
*monitor cells in shaker and move to cold room when OD600 reaches 0.5
*start O/N culture of XL1-blue cells
*aliquot and freeze RNaseH1 protein

Latest revision as of 08:57, 21 April 2009

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The List

  • do ATPase assay on mitochondrial helicase
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