Nelson:To Do - Eric: Difference between revisions
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*streak Xl1-blue onto tet-LB plate <br> | *streak Xl1-blue onto tet-LB plate <br> | ||
*prepare top-agar <br> | *prepare top-agar <br> | ||
*monitor cells in shaker and turn down to 15 deg. C when OD reaches about 0.5 | *monitor cells (your mutant) in shaker and turn down to 15 deg. C when OD reaches about 0.5<br> | ||
Revision as of 10:45, 6 April 2009
The List
- pour 2 SDS-PAGE gels
- prepare DEAE cellulose according to instructions (look for it on protocols page)
- make 1 L of 1 M Tris-HCl, pH 8.0
- 6L of LB and autoclave
- transform polg-a into Rosetta cells
- make tet-LB plates
- streak Xl1-blue onto tet-LB plate
- prepare top-agar
- monitor cells (your mutant) in shaker and turn down to 15 deg. C when OD reaches about 0.5
