Nelson:To Do - Eric

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m (The List)
m (The List)
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*transform your mutant into Rosetta2 cells
*transform your mutant into Rosetta2 cells
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*prepare DEAE cellulose according to instructions (look for it on protocols page)
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*make 1 L of 1 M Tris-HCl, pH 8.0
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*wash out 2 L flasks and make 6L of LB and autoclave

Revision as of 19:19, 2 April 2009

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The List

  • transform your mutant into Rosetta2 cells
  • prepare DEAE cellulose according to instructions (look for it on protocols page)
  • make 1 L of 1 M Tris-HCl, pH 8.0
  • wash out 2 L flasks and make 6L of LB and autoclave
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