Nelson:To Do - Eric: Difference between revisions
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*wash out 2 L flasks and make 6L of LB and autoclave | *wash out 2 L flasks and make 6L of LB and autoclave | ||
*make tet-LB plates <br> | |||
*streak Xl1-blue onto tet-LB plate <br> | |||
*prepare top-agar <br> |
Revision as of 16:22, 2 April 2009
The List
- transform your mutant into Rosetta2 cells
- prepare DEAE cellulose according to instructions (look for it on protocols page)
- make 1 L of 1 M Tris-HCl, pH 8.0
- wash out 2 L flasks and make 6L of LB and autoclave
- make tet-LB plates
- streak Xl1-blue onto tet-LB plate
- prepare top-agar