Nelson:To Do - Eric

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
m (The List)
m (The List)
Line 21: Line 21:
*wash out 2 L flasks and make 6L of LB and autoclave
*wash out 2 L flasks and make 6L of LB and autoclave
 +
 +
*make tet-LB plates <br>
 +
*streak Xl1-blue onto tet-LB plate <br>
 +
*prepare top-agar <br>

Revision as of 19:22, 2 April 2009

Home        Research        Publications        Lab Members        Positions        Contact        Links        Lab stuff       

The List

  • transform your mutant into Rosetta2 cells
  • prepare DEAE cellulose according to instructions (look for it on protocols page)
  • make 1 L of 1 M Tris-HCl, pH 8.0
  • wash out 2 L flasks and make 6L of LB and autoclave
  • make tet-LB plates
  • streak Xl1-blue onto tet-LB plate
  • prepare top-agar
Personal tools