Nelson:To Do - Eric

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m (The List)
m (The List)
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*transform your mutant into Rosetta2 cells
*transform your mutant into Rosetta2 cells
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+
*transform TFAM into Rosetta2 cells
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*pour 1 or 2 SDS-PAGE gels
*prepare DEAE cellulose according to instructions (look for it on protocols page)
*prepare DEAE cellulose according to instructions (look for it on protocols page)

Revision as of 10:12, 3 April 2009

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The List

  • transform your mutant into Rosetta2 cells
  • transform TFAM into Rosetta2 cells
  • pour 1 or 2 SDS-PAGE gels
  • prepare DEAE cellulose according to instructions (look for it on protocols page)
  • make 1 L of 1 M Tris-HCl, pH 8.0
  • wash out 2 L flasks and make 6L of LB and autoclave
  • make tet-LB plates
  • streak Xl1-blue onto tet-LB plate
  • prepare top-agar
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