Nelson:To Do - Eric

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m (The List)
m (The List)
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==The List==
==The List==
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*transform your mutant into Rosetta2 cells
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*transform TFAM into Rosetta2 cells
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*pour 2 SDS-PAGE gels
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*pour 1 or 2 SDS-PAGE gels
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*prepare DEAE cellulose according to instructions (look for it on protocols page)
*prepare DEAE cellulose according to instructions (look for it on protocols page)
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*make 1 L of 1 M Tris-HCl, pH 8.0
*make 1 L of 1 M Tris-HCl, pH 8.0
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*6L of LB and autoclave
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*wash out 2 L flasks and make 6L of LB and autoclave
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*make tet-LB plates <br>
*make tet-LB plates <br>
*streak Xl1-blue onto tet-LB plate <br>
*streak Xl1-blue onto tet-LB plate <br>
*prepare top-agar <br>
*prepare top-agar <br>

Revision as of 17:12, 4 April 2009

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The List

  • pour 2 SDS-PAGE gels
  • prepare DEAE cellulose according to instructions (look for it on protocols page)
  • make 1 L of 1 M Tris-HCl, pH 8.0
  • 6L of LB and autoclave
  • make tet-LB plates
  • streak Xl1-blue onto tet-LB plate
  • prepare top-agar
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