Nelson:To Do - Eric

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m (The List)
m (The List)
Line 19: Line 19:
*make 1 L of 1 M Tris-HCl, pH 8.0
*make 1 L of 1 M Tris-HCl, pH 8.0
*6L of LB and autoclave
*6L of LB and autoclave
 +
*transform polg-a into Rosetta cells
*make tet-LB plates <br>
*make tet-LB plates <br>
*streak Xl1-blue onto tet-LB plate <br>
*streak Xl1-blue onto tet-LB plate <br>
*prepare top-agar <br>
*prepare top-agar <br>
 +
*monitor cells in shaker and turn down to 15 deg. C when OD reaches about 0.5

Revision as of 12:37, 6 April 2009

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The List

  • pour 2 SDS-PAGE gels
  • prepare DEAE cellulose according to instructions (look for it on protocols page)
  • make 1 L of 1 M Tris-HCl, pH 8.0
  • 6L of LB and autoclave
  • transform polg-a into Rosetta cells
  • make tet-LB plates
  • streak Xl1-blue onto tet-LB plate
  • prepare top-agar
  • monitor cells in shaker and turn down to 15 deg. C when OD reaches about 0.5
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