Nelson:To Do - Eric

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
m (The List)
m (The List)
Line 14: Line 14:
==The List==
==The List==
-
 
+
*dishes
*pour 2 SDS-PAGE gels
*pour 2 SDS-PAGE gels
*prepare DEAE cellulose according to instructions (look for it on protocols page)
*prepare DEAE cellulose according to instructions (look for it on protocols page)

Revision as of 13:48, 6 April 2009

Home        Research        Publications        Lab Members        Positions        Contact        Links        Lab stuff       

The List

  • dishes
  • pour 2 SDS-PAGE gels
  • prepare DEAE cellulose according to instructions (look for it on protocols page)
  • make 1 L of 1 M Tris-HCl, pH 8.0
  • 6L of LB and autoclave
  • transform polg-a into Rosetta cells
  • make tet-LB plates
  • streak Xl1-blue onto tet-LB plate
  • prepare top-agar
  • monitor cells (your mutant) in shaker and turn down to 15 deg. C when OD reaches about 0.5
Personal tools