Nelson:To Do - Eric

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==The List==
==The List==
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*dishes
 
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*pour 2 SDS-PAGE gels
 
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*prepare DEAE cellulose according to instructions (look for it on protocols page)
 
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*make 1 L of 1 M Tris-HCl, pH 8.0
 
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*6L of LB and autoclave
 
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*transform polg-a into Rosetta cells
 
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*make tet-LB plates <br>
 
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*streak Xl1-blue onto tet-LB plate <br>
 
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*prepare top-agar <br>
 
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*monitor cells (your mutant) in shaker and turn down to 15 deg. C when OD reaches about 0.5<br>
 

Revision as of 18:40, 6 April 2009

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