Nelson:To Do - Eric

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m (The List)
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==The List==
==The List==
 +
*dishes
 +
*prepare CaCl<sub>2</sub> competent Rosetta pLys cells
 +
*transform RNAp into freshly made competent cells
 +
*monitor cells in shaker and move to cold room when OD600 reaches 0.5
 +
*start O/N culture of XL1-blue cells
 +
*aliquot and freeze RNaseH1 protein

Revision as of 09:01, 8 April 2009

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The List

  • dishes
  • prepare CaCl2 competent Rosetta pLys cells
  • transform RNAp into freshly made competent cells
  • monitor cells in shaker and move to cold room when OD600 reaches 0.5
  • start O/N culture of XL1-blue cells
  • aliquot and freeze RNaseH1 protein
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