Nelson:To Do - Eric: Difference between revisions
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*dishes | *dishes | ||
*prepare CaCl<sub>2</sub> competent Rosetta pLys cells | *prepare CaCl<sub>2</sub> competent Rosetta pLys cells | ||
*make LB-AMP/Chloro plates | |||
*transform RNAp into freshly made competent cells | *transform RNAp into freshly made competent cells | ||
*monitor cells in shaker and move to cold room when OD600 reaches 0.5 | *monitor cells in shaker and move to cold room when OD600 reaches 0.5 | ||
*start O/N culture of XL1-blue cells | *start O/N culture of XL1-blue cells | ||
*aliquot and freeze RNaseH1 protein | *aliquot and freeze RNaseH1 protein | ||
Revision as of 09:27, 8 April 2009
The List
- dishes
- prepare CaCl2 competent Rosetta pLys cells
- make LB-AMP/Chloro plates
- transform RNAp into freshly made competent cells
- monitor cells in shaker and move to cold room when OD600 reaches 0.5
- start O/N culture of XL1-blue cells
- aliquot and freeze RNaseH1 protein
