Nelson:To Do - Eric

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m (The List)
m (The List)
Line 17: Line 17:
*make LB-AMP/Chloro plates
*make LB-AMP/Chloro plates
*transform RNAp into freshly made competent cells
*transform RNAp into freshly made competent cells
 +
*make 4 M NaCl
*monitor cells in shaker and move to cold room when OD600 reaches 0.5
*monitor cells in shaker and move to cold room when OD600 reaches 0.5
*start O/N culture of XL1-blue cells
*start O/N culture of XL1-blue cells
*aliquot and freeze RNaseH1 protein
*aliquot and freeze RNaseH1 protein

Revision as of 12:38, 8 April 2009

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The List

  • dishes
  • prepare CaCl2 competent Rosetta pLys cells
  • make LB-AMP/Chloro plates
  • transform RNAp into freshly made competent cells
  • make 4 M NaCl
  • monitor cells in shaker and move to cold room when OD600 reaches 0.5
  • start O/N culture of XL1-blue cells
  • aliquot and freeze RNaseH1 protein
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