Nelson:To Do - Eric

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==The List==
==The List==
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*dishes
 
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*prepare CaCl<sub>2</sub> competent Rosetta pLys cells
 
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*make LB-AMP/Chloro plates
 
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*transform RNAp into freshly made competent cells
 
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*make 4 M NaCl
 
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*monitor cells in shaker and move to cold room when OD600 reaches 0.5
 
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*start O/N culture of XL1-blue cells
 
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*aliquot and freeze RNaseH1 protein
 

Revision as of 11:59, 9 April 2009

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