Nelson:To Do - Eric

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The List

• dishes
• pour 2 SDS-PAGE gels
• prepare DEAE cellulose according to instructions (look for it on protocols page)
• make 1 L of 1 M Tris-HCl, pH 8.0
• 6L of LB and autoclave
• transform polg-a into Rosetta cells
• make tet-LB plates
  
• streak Xl1-blue onto tet-LB plate
  
• prepare top-agar
  
• monitor cells (your mutant) in shaker and turn down to 15 deg. C when OD reaches about 0.5