Nelson:To Do - Sid

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(The List)
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*make 1 L of 1 M Tris-HCl, pH 8.0
*make 1 L of 1 M Tris-HCl, pH 8.0
*put away dry dishes
*put away dry dishes
 +
*wash out 2 L flasks and make 6L of LB and autoclave

Revision as of 12:22, 2 April 2009

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The List

  • prepare DEAE cellulose according to instructions (look for it on protocols page)
  • rinse centrifuge bottles well and spin down the cells in the 4192 shaker, place in plastic bag, weigh and freeze
  • pour 2 SDS-PAGE gels, 12%
  • make 1 L of 1 M Tris-HCl, pH 8.0
  • put away dry dishes
  • wash out 2 L flasks and make 6L of LB and autoclave
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