Nelson:To Do - Sid

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m (The List)
Line 19: Line 19:
*put away dry dishes
*put away dry dishes
*wash out 2 L flasks and make 6L of LB and autoclave
*wash out 2 L flasks and make 6L of LB and autoclave
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*finish concentrating protein and aliquot into 0.5 ml centrifuge tubes.
+
*finish concentrating protein, read absorbance on nanodrop, aliquot into 0.5 ml centrifuge tubes and freeze in liquid nitrogen.  store in -80oC freezer.

Revision as of 14:02, 2 April 2009

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The List

  • prepare DEAE cellulose according to instructions (look for it on protocols page)
  • rinse centrifuge bottles well and spin down the cells in the 4192 shaker, place in plastic bag, weigh and freeze
  • pour 2 SDS-PAGE gels, 12%
  • make 1 L of 1 M Tris-HCl, pH 8.0
  • put away dry dishes
  • wash out 2 L flasks and make 6L of LB and autoclave
  • finish concentrating protein, read absorbance on nanodrop, aliquot into 0.5 ml centrifuge tubes and freeze in liquid nitrogen. store in -80oC freezer.
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