Nelson:To Do - Sid

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
m (The List)
m (The List)
Line 12: Line 12:
</div>
</div>
==The List==
==The List==
-
*prepare CaCl2 competent Rosetta pLys cells
+
*prepare CaCl<sub>2</sub> competent Rosetta pLys cells
*transform RNAp into freshly made competent cells
*transform RNAp into freshly made competent cells
*spin down the 6L of cells found in cold room
*spin down the 6L of cells found in cold room

Revision as of 09:31, 7 April 2009

Home        Research        Publications        Lab Members        Positions        Contact        Links        Lab stuff       

The List

  • prepare CaCl2 competent Rosetta pLys cells
  • transform RNAp into freshly made competent cells
  • spin down the 6L of cells found in cold room
  • prepare DEAE cellulose according to instructions (look for it on protocols page)
  • make tet-LB plates
  • streak Xl1-blue onto tet-LB plate
  • prepare top-agar
Personal tools