Nelson:To Do - Sid

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m (The List)
m (The List)
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*transform RNAp into freshly made competent cells
*transform RNAp into freshly made competent cells
*spin down the 6L of cells found in cold room
*spin down the 6L of cells found in cold room
 +
*put away dishes
*prepare DEAE cellulose according to instructions (look for it on protocols page)
*prepare DEAE cellulose according to instructions (look for it on protocols page)
*make tet-LB plates <br>
*make tet-LB plates <br>
*streak Xl1-blue onto tet-LB plate <br>
*streak Xl1-blue onto tet-LB plate <br>
*prepare top-agar <br>
*prepare top-agar <br>

Revision as of 10:26, 7 April 2009

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The List

  • prepare CaCl2 competent Rosetta pLys cells
  • transform RNAp into freshly made competent cells
  • spin down the 6L of cells found in cold room
  • put away dishes
  • prepare DEAE cellulose according to instructions (look for it on protocols page)
  • make tet-LB plates
  • streak Xl1-blue onto tet-LB plate
  • prepare top-agar
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