Nelson:To Do - Sid

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m (The List)
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*prepare CaCl<sub>2</sub> competent Rosetta pLys cells
*prepare CaCl<sub>2</sub> competent Rosetta pLys cells
*transform RNAp into freshly made competent cells
*transform RNAp into freshly made competent cells
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*spin down the 6L of cells found in cold room
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*put away dishes
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*prepare DEAE cellulose according to instructions (look for it on protocols page)
*prepare DEAE cellulose according to instructions (look for it on protocols page)
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*make tet-LB plates <br>
 
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*streak Xl1-blue onto tet-LB plate <br>
 
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*prepare top-agar <br>
 

Revision as of 19:06, 7 April 2009

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The List

  • prepare CaCl2 competent Rosetta pLys cells
  • transform RNAp into freshly made competent cells
  • prepare DEAE cellulose according to instructions (look for it on protocols page)
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