Nelson:To Do - Sid

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==The List==
==The List==
 +
*make competent Xl1-blue cells
 +
*transform dsM13 into Xl1-blue
 +
*pour two SDS-PAGE gels (12%)
 +
*run fractions from the polg-b mutant on SDS-PAGE
 +
*spin down the cells in the shaker
 +
*make 6 L of LB and autoclave, plus 4 - 100 ml flasks
 +
*start overnight culture of RNAp (AMP/Chloro)

Revision as of 13:04, 9 April 2009

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The List

  • make competent Xl1-blue cells
  • transform dsM13 into Xl1-blue
  • pour two SDS-PAGE gels (12%)
  • run fractions from the polg-b mutant on SDS-PAGE
  • spin down the cells in the shaker
  • make 6 L of LB and autoclave, plus 4 - 100 ml flasks
  • start overnight culture of RNAp (AMP/Chloro)
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